Occurrence of histone H10-related protein fraction in trout liver

Abstract

The lysine-rich histones of trout liver were studied in order to see whether a protein similar to mammalian histone H1(0) exists in this lower vertebrate. The biochemical methods used involved SDS and acid-urea polyacrylamide gel electrophoresis, gel exclusion and ion-exchange chromatography. Specific rabbit antibodies were elicited against chromatographically-purified mouse liver H1(0) and applied for further characterization of the presumptive trout-liver H1(0) fraction. The results from the indirect immunofluorescence on nitrocellulose-immobilized crude H1 subfractions and from the complement fixation test supplied additional evidence for the existence of an H1(0)-like protein in trout liver.