Enzyme modulator mediated immunoassay (EMMIA)

Abstract

1. Enzyme modulator mediated immunoassay (EMMIA) is a separation-free (homogeneous) enzyme amplified immunoassay. 2. The assay is based on the ability of an analyte labeled enzyme modulator to modify the activity of an indicator enzyme and on ability of an anti-analyte antibody to abrogate the modifying action of the analyte-labeled enzyme modulator upon its binding to anti-analyte antibody. 3. The principle of EMMIA is elaborated in detail. The stages in the development of an EMMIA are described. 4. Criteria in selecting enzymes and modulators suitable for EMMIA are discussed. 5. Examples of EMMIA's using different kinds of modulators for different classes of analytes are presented.