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. 1983 Jul;41(1):430-3.
doi: 10.1128/iai.41.1.430-433.1983.

Isolation and characterization of monoclonal antibodies to Shiga toxin

D E Griffin, M K Gentry, J E Brown

Isolation and characterization of monoclonal antibodies to Shiga toxin

D E Griffin et al. Infect Immun. 1983 Jul.

Abstract

Hybridoma cell lines which produce monoclonal antibodies to Shiga toxin from Shigella dysenteriae 1 were prepared. The monoclonal antibodies were all of the immunoglobulin G1 isotype and differed in their ability to neutralize cytotoxicity and to bind to Shiga toxin in a solid-phase radioimmunoassay. When used for immunoblot analysis, these antibodies were able to identify specifically both nicked and unnicked Shiga toxin in crude lysates of S. dysenteriae.

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References

    1. Infect Immun. 1982 Jun;36(3):996-1005 - PubMed
    1. Infect Immun. 1980 Jul;29(1):98-107 - PubMed
    1. J Biol Chem. 1981 Aug 25;256(16):8739-44 - PubMed
    1. Infect Immun. 1982 Mar;35(3):1151-4 - PubMed
    1. J Clin Invest. 1972 May;51(5):1212-8 - PubMed

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