Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Jul;47(1):227-32.
doi: 10.1128/JVI.47.1.227-232.1983.

Construction of an infectious molecular clone of the autonomous parvovirus minute virus of mice

M J Merchlinsky, P J Tattersall, J J Leary, S F Cotmore, E M Gardiner, D C Ward

Construction of an infectious molecular clone of the autonomous parvovirus minute virus of mice

M J Merchlinsky et al. J Virol. 1983 Jul.

Abstract

The linear single-stranded DNA genome of minute virus of mice, an autonomous parvovirus, was cloned in duplex form into the bacterial plasmid pBR322. The recombinant clones of minute virus of mice were infectious when transfected into monolayers of human 324K cells and produced virus plaques with an efficiency of about 6% that obtained with duplex replicative-form DNA purified from cells infected with minute virus of mice. Southern blot analysis of transfected cells indicated that the cloned minute virus of mice genome requires both termini to be intact for excision and replication as a linear duplex molecule.

PubMed Disclaimer

References

    1. J Mol Biol. 1967 Jun 14;26(2):365-9 - PubMed
    1. J Mol Biol. 1969 May 14;41(3):459-72 - PubMed
    1. Virology. 1973 Apr;52(2):456-67 - PubMed
    1. J Virol. 1973 Dec;12(6):1446-56 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 - PubMed

Publication types

LinkOut - more resources