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. 1983:93:3-12.
doi: 10.1016/s0076-6879(83)93031-8.

Production of antibodies by inoculation into lymph nodes

M B Sigel, Y N Sinha, W P VanderLaan

Production of antibodies by inoculation into lymph nodes

M B Sigel et al. Methods Enzymol. 1983.

Abstract

The intra-lymph node technique used to inoculate rabbits with small quantities of antigen has been described. A variety of antigens in the 20,000-22,000 molecular weight range, as well as a 15-amino acid peptide coupled to BSA, have been inoculated successfully by this procedure. We have made no attempt to compare the success rate of the intra-lymph node inoculation route with other techniques utilizing small (microgram) quantities of antigen.

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