doi: 10.1128/jb.155.2.488-492.1983.
Oxygen requirements for formation and activity of the squalene epoxidase in Saccharomyces cerevisiae
- PMID: 6348021
- PMCID: PMC217714
- DOI: 10.1128/jb.155.2.488-492.1983
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Oxygen requirements for formation and activity of the squalene epoxidase in Saccharomyces cerevisiae
J Bacteriol.
1983 Aug.
Abstract
The effect of oxygen on squalene epoxidase activity in Saccharomyces cerevisiae was investigated. In cells grown in standing cultures, the epoxidase was localized mainly in the "mitochondrial" fraction. Upon aeration, enzyme activity increased and the newly formed enzyme was associated with the "microsomal" fraction. At 0.03% (vol/vol) oxygen, epoxidase levels doubled, whereas the ergosterol level was only slightly increased. Cycloheximide inhibited the increase in epoxidase under these conditions. An apparent Km for oxygen of 0.38% (vol/vol) was determined from a crude particulate preparation for the epoxidase.
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