Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Aug;155(2):488-92.
doi: 10.1128/jb.155.2.488-492.1983.

Oxygen requirements for formation and activity of the squalene epoxidase in Saccharomyces cerevisiae

L Jahnke, H P Klein

Oxygen requirements for formation and activity of the squalene epoxidase in Saccharomyces cerevisiae

L Jahnke et al. J Bacteriol. 1983 Aug.

Abstract

The effect of oxygen on squalene epoxidase activity in Saccharomyces cerevisiae was investigated. In cells grown in standing cultures, the epoxidase was localized mainly in the "mitochondrial" fraction. Upon aeration, enzyme activity increased and the newly formed enzyme was associated with the "microsomal" fraction. At 0.03% (vol/vol) oxygen, epoxidase levels doubled, whereas the ergosterol level was only slightly increased. Cycloheximide inhibited the increase in epoxidase under these conditions. An apparent Km for oxygen of 0.38% (vol/vol) was determined from a crude particulate preparation for the epoxidase.

PubMed Disclaimer

References

    1. Biochem Biophys Res Commun. 1981 Nov 16;103(1):272-7 - PubMed
    1. Biochim Biophys Acta. 1970 Dec 16;220(3):463-8 - PubMed
    1. J Biol Chem. 1957 Jun;226(2):931-9 - PubMed
    1. Biochim Biophys Acta. 1954 Apr;13(4):591 - PubMed
    1. J Bacteriol. 1967 Jul;94(1):61-5 - PubMed

LinkOut - more resources