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. 1983 Jun;45(6):1838-47.
doi: 10.1128/aem.45.6.1838-1847.1983.

Improved conversion of fumarate to succinate by Escherichia coli strains amplified for fumarate reductase

I Goldberg, K Lonberg-Holm, E A Bagley, B Stieglitz

Improved conversion of fumarate to succinate by Escherichia coli strains amplified for fumarate reductase

I Goldberg et al. Appl Environ Microbiol. 1983 Jun.

Abstract

Two recombinant plasmid Escherichia coli strains containing amplified fumarate reductase activity converted fumarate to succinate at significantly higher rates and yields than a wild-type E. coli strain. Glucose was required for the conversion of fumarate to succinate, and in the absence of glucose or in cultures with a low cell density, malate accumulated. Two-dimensional gel electrophoretic analysis of proteins from the recombinant DNA and wild-type strains showed that increased quantities of both large and small fumarate reductase subunits were expressed in the recombinant DNA strains.

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