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Comparative Study
. 1983 Oct;54(1):163-8.

Circulating antibodies to the surface antigens on colon epithelial cells in ulcerative colitis

T Hibi, S Aiso, M Ishikawa, M Watanabe, T Yoshida, K Kobayashi, H Asakura, S Tsuru, M Tsuchiya
Comparative Study

Circulating antibodies to the surface antigens on colon epithelial cells in ulcerative colitis

T Hibi et al. Clin Exp Immunol. 1983 Oct.

Abstract

The fluorescence activated cell sorter (FACS) was used for detecting circulating antibodies to the surface antigens on isolated colon epithelial cells (anti-colon antibodies) by indirect immunofluorescence. Anti-colon antibodies were found in the serum of 30 of 41 (73%) patients with ulcerative colitis. This incidence is much higher than one established in earlier reports by application of indirect immunofluorescence to colon tissue using the fluorescence microscope. The results suggest that FACS analysis is very useful for detecting antibodies to colon specific antigen.

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