D2-protein in PC12 pheochromocytoma cells after nerve growth factor stimulation

Abstract

The rat brain D2-protein has previously been shown to play a role in interneuronal adhesion Monospecific antisera against this protein reacted with adrenal medulla cells and with PC12 pheochromocytoma cells as demonstrated by indirect immunofluorescence. Upon stimulation by nerve growth factor the PC12 cells extend neurites. These neurites were shown to contain D2-protein both by immunofluorescence and by crossed immunoelectrophoresis. The findings substantiate the close relationship between neurons from the central nervous system and both the PC12 cells line, as well as adrenal medulla cells.