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. 1983 Nov 11;64(1-2):179-87.
doi: 10.1016/0022-1759(83)90396-4.

An ELISA method for quantitation of macrophage migration from agarose microdroplets

R Peck

An ELISA method for quantitation of macrophage migration from agarose microdroplets

R Peck. J Immunol Methods. .

Abstract

A method is described for quantitating the biological activity of macrophage migration inhibitory factor (MIF) based on the detection of macrophages migrating from agarose droplets. The migration is determined with polyclonal anti-macrophage antibodies in an ELISA assay. The numbers of macrophages thus detected correlate well with the migration as measured by a capillary tube assay for MIF.

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