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. 1983 Nov;46(5):1146-51.
doi: 10.1128/aem.46.5.1146-1151.1983.

DNA-DNA hybridization assay for detection of Salmonella spp. in foods

R Fitts, M Diamond, C Hamilton, M Neri

DNA-DNA hybridization assay for detection of Salmonella spp. in foods

R Fitts et al. Appl Environ Microbiol. 1983 Nov.

Abstract

We have developed a DNA-DNA hybridization test for the presence of Salmonella spp. in foods. This test requires an initial pre-enrichment of food samples in nutrient broth but does not require selective enrichment. Samples of food cultures are collected on membrane filters and assayed by molecular hybridization to labeled probes. The probes consist of DNA sequences which are unique to the genus Salmonella and are widely distributed in the genus. A diverse panel of foods was assayed successfully by this methodology.

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