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Comparative Study
. 1984 Jan;19(1):81-2.
doi: 10.1128/jcm.19.1.81-82.1984.

Lyme disease: a selective medium for isolation of the suspected etiological agent, a spirochete

S E Johnson, G C Klein, G P Schmid, G S Bowen, J C Feeley, T Schulze
Comparative Study

Lyme disease: a selective medium for isolation of the suspected etiological agent, a spirochete

S E Johnson et al. J Clin Microbiol. 1984 Jan.

Abstract

A simple procedure with a new selective culture medium for the isolation of the suspected etiological agent of Lyme disease from ticks is described. Live ticks (Ixodes dammini) were ground with a mortar and pestle, and the suspensions were inoculated into a selective and nonselective medium. The selective medium, which contained kanamycin and 5-fluorouracil, yielded positive spirochete cultures from 100% of the pooled ticks and from 79% of the single tick specimens. The isolation rate for the nonselective medium was 0% from the tick pools and 58% from the single tick specimens.

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