Elimination of artifactual acetaldehyde in the measurement of human blood acetaldehyde by the use of polyethylene glycol and sodium azide: normal blood acetaldehyde levels in the dog and human after ethanol

Abstract

A new procedure is described for the preparation of human blood samples for analysis of acetaldehyde and ethanol by head space gas chromatography. High concentrations of polyethylene glycol were used to remove the hemoglobin and approximately 50% of the plasma protein. Artifactual formation of acetaldehyde from ethanol was inhibited by sodium azide. Using this method, no artifactual acetaldehyde was detectable in human, dog, sheep, and rat blood when spiked with ethanol in final concentrations of 65 mM. The recovery of added acetaldehyde was approximately 80% for human dog, and sheep blood, whereas it was only 30% for rat blood. Following ethanol administration, acetaldehyde levels were determined in blood taken from the pulmonary artery and descending aorta of the dog and human, and also from the hepatic vein of the latter. The relative blood acetaldehyde concentrations at these sites were hepatic vein greater than pulmonary artery greater than descending aorta.