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. 1983 Oct;13(2):143-8.
doi: 10.1016/0045-6039(83)90106-9.

Stabilization of the maturation promoting factor (MPF) from Xenopus laevis oocytes. Protection against calcium ions

J Hermann, R Bellé, J Tso, R Ozon

Stabilization of the maturation promoting factor (MPF) from Xenopus laevis oocytes. Protection against calcium ions

J Hermann et al. Cell Differ. 1983 Oct.

Abstract

Maturation promoting factor (MPF) activity was recovered from progesterone-matured Xenopus oocytes cytosol, fractionated by polyethylene glycol-20,000 or ethanolic precipitation. An improved stabilization of the biological activity was obtained by adding adenosine 5'-O-(3-thiotriphosphate) (ATP-gamma-S) (50 microM) to the preparation buffers. Neither Ca2+ ions nor calmodulin inhibit the partially purified MPF.

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