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. 1983 Nov;25(2-3):333-41.
doi: 10.1016/0378-1119(83)90238-x.

A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast

R J Klebe, J V Harriss, Z D Sharp, M G Douglas

A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast

R J Klebe et al. Gene. 1983 Nov.

Abstract

Polyethylene glycol (PEG) can induce genetic transformation in both bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) without cell wall removal. PEG-mediated transformation of E. coli is technically simple and yields transformants with an efficiency of 10(6)-10(7) transformants/microgram DNA. Detailed analysis of the parameters involved in PEG-mediated transformation of E. coli reveals basic differences between the PEG and standard CaCl2 methods for transformation of E. coli. PEG-mediated transformation of yeast is far simpler than existing protoplast methods and is comparable in efficiency. The new methods described here for PEG-mediated genetic transformation may prove to be of general utility in performing genetic transformation in a wide variety of organisms.

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