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. 1984 Jan 11;12(1 Pt 2):789-800.
doi: 10.1093/nar/12.1part2.789.

Escherichia coli promoter sequences predict in vitro RNA polymerase selectivity

M E Mulligan, D K Hawley, R Entriken, W R McClure
Free PMC article

Escherichia coli promoter sequences predict in vitro RNA polymerase selectivity

M E Mulligan et al. Nucleic Acids Res. .
Free PMC article

Abstract

We describe a simple algorithm for computing a homology score for Escherichia coli promoters based on DNA sequence alone. The homology score was related to 31 values, measured in vitro, of RNA polymerase selectivity, which we define as the product KBk2, the apparent second order rate constant for open complex formation. We found that promoter strength could be predicted to within a factor of +/-4.1 in KBk2 over a range of 10(4) in the same parameter. The quantitative evaluation was linked to an automated (Apple II) procedure for searching and evaluating possible promoters in DNA sequence files.

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References

    1. Annu Rev Genet. 1979;13:319-53 - PubMed
    1. Cell. 1980 Jun;20(2):269-81 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5634-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6381-5 - PubMed
    1. Nucleic Acids Res. 1982 Jan 11;10(1):39-49 - PubMed

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