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. 1984 Jan;81(2):409-13.
doi: 10.1073/pnas.81.2.409.

tRNAMetf2 gene in the leader region of the nusA operon in Escherichia coli

S Ishii, K Kuroki, F Imamoto

tRNAMetf2 gene in the leader region of the nusA operon in Escherichia coli

S Ishii et al. Proc Natl Acad Sci U S A. 1984 Jan.

Abstract

The promoter-proximal portion of the operon containing the Escherichia coli nusA gene has been cloned. Its nucleotide sequence shows that genes for tRNAMetf2 and a 15-kilodalton protein of unknown function precede the nusA protein gene. The sequence suggests that the three genes form a single transcription unit. Consistent with this hypothesis, purified RNA polymerase formed full-length transcripts on the cloned DNA in vitro, although transcription was frequently arrested at the intercistronic site(s) between the gene for tRNAMetf2 and the 15-kilodalton protein.

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References

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