One-step Plasmodium falciparum cultivation--application to in-vitro drug testing
- PMID: 6364513
One-step Plasmodium falciparum cultivation--application to in-vitro drug testing
Abstract
P. falciparum was cultivated by a one-step method which requires medium renewal and addition of fresh RBC only every three days and allows high proliferation rates of the parasite. The method has been used for routine maintenance of strains and applied to the in-vitro evaluation of the effect of antimalarial compounds over 3 days periods as measured by 3H-Hypoxanthine incorporation.
Similar articles
-
[In vitro cultivation of Plasmodium falciparum. Applications and limits.- Methodology].Med Trop (Mars). 1982 Jul-Aug;42(4):437-62. Med Trop (Mars). 1982. PMID: 6755144 Review. French.
-
Effect of tissue culture media on multiplication of Plasmodium falciparum in vitro.Indian J Malariol. 1992 Jun;29(2):73-82. Indian J Malariol. 1992. PMID: 1459308
-
Simple in vitro cultivation of the malaria parasite Plasmodium falciparum (erythrocytic stages) suitable for large-scale preparations.Methods Mol Biol. 1993;21:43-55. doi: 10.1385/0-89603-239-6:43. Methods Mol Biol. 1993. PMID: 8220733 No abstract available.
-
An in vitro assay system for the identification of potential antimalarial drugs.J Parasitol. 1983 Jun;69(3):577-83. J Parasitol. 1983. PMID: 6355423
-
Plasmodium falciparum biology: analysis of in vitro versus in vivo growth conditions.Trends Parasitol. 2009 Oct;25(10):474-81. doi: 10.1016/j.pt.2009.07.005. Epub 2009 Sep 9. Trends Parasitol. 2009. PMID: 19747879 Review.
Cited by
-
Activity of a combination of three cinchona bark alkaloids against Plasmodium falciparum in vitro.Antimicrob Agents Chemother. 1988 Feb;32(2):250-4. doi: 10.1128/AAC.32.2.250. Antimicrob Agents Chemother. 1988. PMID: 3284455 Free PMC article.
-
The development of malaria diagnostic techniques: a review of the approaches with focus on dielectrophoretic and magnetophoretic methods.Malar J. 2016 Jul 12;15(1):358. doi: 10.1186/s12936-016-1400-9. Malar J. 2016. PMID: 27405995 Free PMC article. Review.
-
Cultivation of Asexual Intraerythrocytic Stages of Plasmodium falciparum.Pathogens. 2023 Jul 1;12(7):900. doi: 10.3390/pathogens12070900. Pathogens. 2023. PMID: 37513747 Free PMC article. Review.