Fluorescence immunoassay

Abstract

Fluorescence immunoassay is a sensitive technique that can be used in the measurement of many compounds, including drugs, hormones, and proteins; in the identification of antibodies; and in the quantification of antigens such as viral particles and, potentially, bacteria. Homogeneous fluorescence immunoassay, fluorescent excitation transfer immunoassay, fluorescence polarization immunoassay, solid-phase "dipstick" immunoassay, solid-phase microbead fluorescence immunoassay, substrate-labeled fluorescence immunoassay, and fluorescence immunoassays using internal reflectance spectroscopy or phycobiliprotein conjugates are reviewed.