Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Nov 25;64(3):345-51.
doi: 10.1016/0022-1759(83)90442-8.

Maintenance of granuloma macrophages in serum-free medium

R M Fauve, H Jusforgues, B Hevin

Maintenance of granuloma macrophages in serum-free medium

R M Fauve et al. J Immunol Methods. .

Abstract

Granulomas were induced by injecting polyacrylamide beads into subcutaneous pouches created by divulsion of the dorsal skin of mice. More than 10(7) phagocytic cells (60% macrophages, 40% polymorphonuclear cells) could be recovered from this granuloma. The separation of the phagocytic cells can be achieved either following sedimentation in Percoll or following the incubation of cells on plastic petri dishes. Phagocytosis of zymosan was observed in macrophages maintained in vitro for 1 month in Eagle's serum-free medium.

PubMed Disclaimer

Publication types

LinkOut - more resources