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. 1984 Apr;158(1):365-8.
doi: 10.1128/jb.158.1.365-368.1984.

Cloning of the Escherichia coli release factor 2 gene

C T Caskey, W C Forrester, W Tate, C D Ward

Cloning of the Escherichia coli release factor 2 gene

C T Caskey et al. J Bacteriol. 1984 Apr.

Abstract

The protein release factor 2 (RF2) participates in Escherichia coli polypeptide chain termination with codon specificity (UAA or UGA). A colicin E1 recombinant identified in the Carbon and Clarke E. coli bank contains the protein release factor 2 gene. A 1.7-kilobase E. coli fragment has been subcloned into the plasmid pUC9 vector. Bacterial cells, containing the plasmid recombinant, produce elevated levels of protein release factor 2 as detected by an immune precipitation assay and in vitro measurement of UGA-directed peptide chain termination and [3H]UGA codon recognition.

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