Collaborative clinical evaluation of the Autobac IDX system for identification of gram-negative bacilli

Abstract

The Autobac IDX system (General Diagnostics, Warner-Lambert Co., Morris Plains, N.J.) for rapid, semiautomated identification of gram-negative bacilli was compared with the identification methods in routine use in four laboratories. The study included 1,515 organisms representing 30 species of enteric and nonenteric bacteria. Discrepancies between the results of the IDX system and routine methods were resolved by classical biochemical testing at a reference center. Overall, 98% of the organisms were correctly identified by the routine methods, and 93% were correctly identified by the IDX systems. After adjustment for frequency of clinical occurrence of the organisms tested, the IDX system performed with 95% accuracy. Results with the IDX system were available in 3 to 6 h. Results with the comparative methods were available in 4 to 48 h. A wide variety of organisms, including oxidase positive, oxidase negative, fermentative, and nonfermentative, were identified by a single system by using Autobac. Three or more systems were required to identify the 30 species by the comparative methods. Overall, the results indicate the Autobac IDX system is useful for the rapid identification of enteric and nonfermentative gram-negative bacilli.