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. 1984 May;158(2):737-8.
doi: 10.1128/jb.158.2.737-738.1984.

Expression of a Thiobacillus ferrooxidans origin of replication in Escherichia coli

D E Rawlings, I Pretorius, D R Woods

Expression of a Thiobacillus ferrooxidans origin of replication in Escherichia coli

D E Rawlings et al. J Bacteriol. 1984 May.

Abstract

A cryptic plasmid from an autotrophically grown arsenic-resistant strain of Thiobacillus ferrooxidans was isolated and cloned into pBR325. The origin of replication of pBR325 was deleted, and the recombinant plasmid was shown to replicate in Escherichia coli, using an origin of replication located on the Thiobacillus plasmid.

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References

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