5-Methylcytosine is not detectable in Saccharomyces cerevisiae DNA
- PMID: 6374428
- PMCID: PMC368855
- DOI: 10.1128/mcb.4.5.985-988.1984
5-Methylcytosine is not detectable in Saccharomyces cerevisiae DNA
Abstract
We examined the DNA of Saccharomyces cerevisiae by both HpaII-MspI restriction enzyme digestion and high-performance liquid chromatography analysis for the possible presence of 5-methylcytosine. Both of these methods failed to detect cytosine methylation within this yeast DNA; i.e., there is less than 1 5-methylcytosine per 3,100 to 6,000 cytosine residues.
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