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. 1984 Jun;81(11):3466-70.
doi: 10.1073/pnas.81.11.3466.

Chemically modified nucleic acids as immunodetectable probes in hybridization experiments

P Tchen, R P Fuchs, E Sage, M Leng

Chemically modified nucleic acids as immunodetectable probes in hybridization experiments

P Tchen et al. Proc Natl Acad Sci U S A. 1984 Jun.

Abstract

Guanine residues in nucleic acids can be modified by treatment with N-acetoxy-N-2-acetylaminofluorene and its 7-iodo derivative in an in vitro nonenzymatic reaction. The modified nucleic acids (ribo or deoxyribo, single or double stranded) are recognized by specific antibodies. They can be immunoprecipitated or used as probes in hybridization experiments and detected by immunochemical techniques.

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