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. 1984 May;134(Pt 2):169-71.
doi: 10.1111/j.1365-2818.1984.tb02506.x.

Ethanol, an efficient coolant for rapid freezing of biological material

S P Marchese-Ragona

Ethanol, an efficient coolant for rapid freezing of biological material

S P Marchese-Ragona. J Microsc. 1984 May.

Abstract

Supercooled ethanol was used for freeze-quenching protozoan samples and gave reasonable ultrastructural preservation. The use of ethanol as a coolant has considerable advantages in terms of its relative safety, high cooling efficiency and low cost when compared with other coolants currently used for freeze-quenching.

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