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Comparative Study
. 1984 Jun 25;12(12):4997-5003.
doi: 10.1093/nar/12.12.4997.

Structure and function of tryptophan tRNA from wheat germ

K Ghosh, H P Ghosh
Free PMC article
Comparative Study

Structure and function of tryptophan tRNA from wheat germ

K Ghosh et al. Nucleic Acids Res. .
Free PMC article

Abstract

The coding properties of tRNATrp from yeast and wheat germ were studied. Unlike E. coli tRNATrp or mitochondrial tRNATrp, eukaryotic tRNATrp did not recognize the UGA codon in vitro. The sequence of wheat germ tRNATrp as determined by [32P] post-labelling techniques is: [sequence in text] The interesting features are: (i) Presence of a C11:G24 base pair in contrast to the U11:G24 in E. coli Su- tRNATrp. (ii) The anticodon sequence is -CmCA- compared to -CCA- in E. coli tRNATrp. (iii) Lack of a hypermodified base i6A adjacent to the 3'-end of the anticodon. (iv) Presence of -T psi CG- sequence instead of -psi psi CG- sequence present in mammalian tRNATrp.

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References

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