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. 1984 May 15;139(1):100-3.
doi: 10.1016/0003-2697(84)90394-4.

Extraction of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis from protease-rich plant tissues

F S Wu, M Y Wang

Extraction of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis from protease-rich plant tissues

F S Wu et al. Anal Biochem. .

Abstract

A method of extracting proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis from plant tissues with high protease activity was described. It resolved protein bands in high-molecular-weight regions of the gel and replaced commonly used procedures which showed severe degradation of proteins, even in the presence of protease inhibitors.

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