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. 1984 Jul;3(7):1609-12.
doi: 10.1002/j.1460-2075.1984.tb02018.x.

RNA secondary structure and translation inhibition: analysis of mutants in the rplJ leader

T Christensen, M Johnsen, N P Fiil, J D Friesen

RNA secondary structure and translation inhibition: analysis of mutants in the rplJ leader

T Christensen et al. EMBO J. 1984 Jul.

Abstract

We have carried out measurements of the stable binding of the ribosomal protein (r-protein) complex L10-L7/L12 to mutant forms of the mRNA leader of the rplJ operon of Escherichia coli. One of the point mutations, base 1548, which lies within the L10-L7/L12-protected region, almost completely abolishes in vitro formation of a stable complex of L10-L7/L12 with rplJ mRNA leader, and a second point mutation, base 1634, strongly reduces it. These observations constitute strong support for the proposition that L10-L7/L12 binds to the rplJ leader in bringing about translational feedback. To account for the action of these and other mutations, and to explain the mechanism of translation feedback inhibition, we suggest a secondary structure model involving alternate forms of the rplJ mRNA leader.

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