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. 1984 Jul 23;173(1):108-12.
doi: 10.1016/0014-5793(84)81027-3.

Expression of cloned cDNA for a major surface antigen of Plasmodium falciparum merozoites

K G Odink, M J Lockyer, S C Nicholls, Y Hillman, R R Freeman, A A Holder
Free article

Expression of cloned cDNA for a major surface antigen of Plasmodium falciparum merozoites

K G Odink et al. FEBS Lett. .
Free article

Abstract

A cDNA library of P. falciparum was constructed. Using size-selected mRNA as a probe several clones were isolated which hybridized to mRNAs larger than 5 kilobases (kb). The cDNA insert of pFC 17, which hybridizes to 5.6-kb mRNA was expressed by fusion to anthranilate synthetase I in a plasmid expression vector. The expressed fusion protein was shown to contain epitopes of a 195-kDa protein which is the precursor to 3 major surface antigens of P. falciparum merozoites.

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