Expulsion of Trichinella spiralis from the intestine of W/Wv mice reconstituted with haematopoietic and lymphopoietic cells and origin of mucosal mast cells

Abstract

The involvement of haematopoietic and lymphopoietic cells in the expulsion of Trichinella spiralis from the intestine of mice, and the origin of the subepithelial mast cells (SMCs) and the globule leucocytes (GLs) were determined using SMC- and GL-deficient WBB6F1-W/Wv and WCB6F1-S1/S1d mice. On day 12 after infection with T. spiralis, SMCs and GLs appeared in W/Wv mice injected with spleen and bone marrow cells from normal +/+ mice, but not in W/Wv mice injected with thymus, mesenteric lymph node and Peyer's patch cells from +/+ mice or in S1/S1d mice injected with spleen cells from normal mice. On day 22 after infection, GLs and SMCs with giant granules were found in W/Wv mice injected with spleen cells of C57BL/6J-bgJ/bgJ mice in which GLs and SMCs contained giant granules. These results suggested that the precursor cells of SMCs and GLs were located in the haematopoietic tissues. In W/Wv mice reconstituted with SMCs and GLs, expulsion of T. spiralis from the intestine was as rapid as that of the normal mice, but in W/Wv mice injected with lymphopoietic cells and S1/S1d mice which had been injected with normal spleen cell, expulsion of T. spiralis was less rapid. The result suggested that haematopoietic cells containing SMC- and GL-precursor cells might be involved in the expulsion of T. spiralis from the intestine of mice.