doi: 10.1016/0003-2697(84)90054-x.
An improved bulk purification method for Escherichia coli elongation factor, Ts
- PMID: 6388412
- DOI: 10.1016/0003-2697(84)90054-x
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An improved bulk purification method for Escherichia coli elongation factor, Ts
Anal Biochem.
1984 Sep.
Abstract
A bulk purification procedure has been designed to maximize the yield of Escherichia coli elongation factor, Ts, with a minimum of effort and time. The enzyme purification is achieved by DEAE-Sepharose and elongation factor Tu-affinity chromatographies. The typical yield is 150 mg/kg of E. coli (B) cells.
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