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. 1984 Sep;141(2):351-4.
doi: 10.1016/0003-2697(84)90054-x.

An improved bulk purification method for Escherichia coli elongation factor, Ts

M Yoder, J Lennane, F Jurnak

An improved bulk purification method for Escherichia coli elongation factor, Ts

M Yoder et al. Anal Biochem. 1984 Sep.

Abstract

A bulk purification procedure has been designed to maximize the yield of Escherichia coli elongation factor, Ts, with a minimum of effort and time. The enzyme purification is achieved by DEAE-Sepharose and elongation factor Tu-affinity chromatographies. The typical yield is 150 mg/kg of E. coli (B) cells.

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