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. 1984 Sep;141(2):402-8.
doi: 10.1016/0003-2697(84)90061-7.

Affinity purification of aminoacyl-tRNA

A Louie, E Masuda, M Yoder, F Jurnak

Affinity purification of aminoacyl-tRNA

A Louie et al. Anal Biochem. 1984 Sep.

Abstract

A procedure for separating Escherichia coli aminoacyl-tRNA from unacylated tRNA or components of the aminoacylation reaction, thereby achieving an aminoacyl-tRNA product with a very high specific activity, is described. The method utilizes the specific recognition of aminoacyl-tRNA for E. coli protein synthesis elongation factor Tu which has been immobilized on an affinity matrix. The application of the affinity procedure as a means of purifying a single aminoacyl-tRNA from an unfractionated mixture of tRNAs is also discussed.

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