[Primary structure of the E. coli DNA-dependent RNA-polymerase beta'-subunit. Hydrolysis with trypsin]

Abstract

The carboxymethylated beta'-subunit of DNA-dependent RNA polymerase was hydrolyzed with trypsin. The hydrolysate was separated on Bio-Gel P-4, followed by ion exchange chromatography, and was further purified by paper chromatography and electrophoresis. A mixture of large peptides was digested with Staphylococcus aureus protease, the fragments obtained were separated by an HPLC procedure. As a result, 172 peptides were isolated, the complete amino acid sequence for 162 and partial sequence for 10 of them being determined. In total, these peptides contain 862 amino acid residues.