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. 1978 Apr;24(4):436-9.
doi: 10.1139/m78-072.

Double-antibody radioimmunoassay for staphylococcal enterotoxins A and B

H Robern, T M Gleeson, R A Szabo

Double-antibody radioimmunoassay for staphylococcal enterotoxins A and B

H Robern et al. Can J Microbiol. 1978 Apr.

Abstract

A sensitive double-antibody radioimmunoassay for staphylococcal enterotoxins A and B is described. The separation of the primary antigen-antibody complex of enterotoxin A and B was achieved with an anti-rabbit gamma globulin from goats. Radioiodinated aggregate fractions of staphylococcal enterotoxins exhibited reduced immunological activity and showed little competition with non-radioactive exterotoxin. The radioimmunoassay was successfully applied for the quantitation of enterotoxins in food.

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