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. 1984 Dec;155(2):588-92.
doi: 10.1016/0014-4827(84)90219-2.

Synaptonemal complex-associated centromeres and recombination nodules in plant meiocytes prepared by an improved surface-spreading technique

S M Albini, G H Jones

Synaptonemal complex-associated centromeres and recombination nodules in plant meiocytes prepared by an improved surface-spreading technique

S M Albini et al. Exp Cell Res. 1984 Dec.

Abstract

An improved method of preparing two-dimensional surface spreads of plant synaptonemal complexes (SCs) is described. This technique produces clear preparations of SCs and, in addition, consistently reveals both centromeres and recombination nodules (RNs) in PTA-stained preparations viewed by electron microscopy. A preliminary study of RN number and distribution in Allium fistulosum indicates that they faithfully reflect the positions of cross-over exchange events as revealed by chiasmata.

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