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. 1984 Nov;46(2):465-9.
doi: 10.1128/iai.46.2.465-469.1984.

Oral vaccination of monkeys with an invasive Escherichia coli K-12 hybrid expressing Shigella flexneri 2a somatic antigen

S B Formal, T L Hale, C Kapfer, J P Cogan, P J Snoy, R Chung, M E Wingfield, B L Elisberg, L S Baron

Oral vaccination of monkeys with an invasive Escherichia coli K-12 hybrid expressing Shigella flexneri 2a somatic antigen

S B Formal et al. Infect Immun. 1984 Nov.

Abstract

A living oral vaccine, designed to protect against Shigella flexneri 2a infections, was constructed by using Escherichia coli K-12 as a carrier strain. The hybrid strain, designated EC104, contained both chromosomal and plasmid genes from S. flexneri donor strains. In addition to expressing the S. flexneri 2a somatic antigen, it had inherited the property of epithelial-cell invasion. After the oral administration to rhesus monkeys, EC104 was isolated from the feces for up to 3 days, but by day 4 all stool cultures were negative. The serum antibody response against S. flexneri 2a somatic antigen was variable, but the vaccine conferred significant protection against an oral challenge with virulent S. flexneri 2a.

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References

    1. J Infect Dis. 1977 Oct;136(4):577-82 - PubMed
    1. J Bacteriol. 1965 Jul;90(1):63-8 - PubMed
    1. J Bacteriol. 1980 Aug;143(2):926-33 - PubMed
    1. Am J Trop Med Hyg. 1955 Mar;4(2):281-8 - PubMed
    1. Bull World Health Organ. 1965;32(5):647-55 - PubMed

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