Cloning of the methionine regulatory gene, metJ, of Escherichia coli K12 and identification of its product

Abstract

Both wild-type and mutant forms of the methionine regulatory gene, metJ, of Escherichia coli K12 have been cloned in derivatives of pBR322. In cells carrying plasmids with a functional copy of metJ, the methionine regulon appears to be repressed even under conditions of methionine limitation. Maxicell labeling experiments show that the plasmids code for a small peptide (12 kilodaltons) only when they carry a functional copy of metJ. The lesions in five independently isolated metJ mutants are located in, or slightly upstream from, a coding sequence proposed to be metJ by Saint-Girons, I., Duchange, N., Cohen, G. N., and Zakin, M. M. [1984) J. Biol. Chem. 259, 14282-14285).