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. 1984 Nov;33(6):1051-4.
doi: 10.4269/ajtmh.1984.33.1051.

Monoclonal antibody characterization of Plasmodium falciparum antigens

G H Campbell, L H Miller, D Hudson, E L Franco, P M Andrysiak

Monoclonal antibody characterization of Plasmodium falciparum antigens

G H Campbell et al. Am J Trop Med Hyg. 1984 Nov.

Abstract

We characterized a set of eight monoclonal antibodies produced against Plasmodium falciparum. In an indirect fluorescent antibody assay the antibodies produced small dots of fluorescence in schizonts and individual merozoites. This merozoite-associated dot reactivity occurred with 21 different strains of P. falciparum, but there was no reactivity with other human, nonhuman primate, or rodent Plasmodium species. Three of the monoclonal antibodies precipitated proteins of Mr 145,000, 135,000, and 104,000. Five of the monoclonal antibodies precipitated proteins of Mr 78,000, 63,000, 42,000, and 40,000.

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