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Comparative Study
. 1984 Oct 30;88(1):31-7.
doi: 10.1007/BF00439292.

Enzyme-linked immunosorbent assay (ELISA) in the paracoccidioidomycosis. Comparison with counterimmunoelectrophoresis and erythro-immunoassay

Z P de Camargo, J L Guesdon, E Drouhet, L Improvisi
Comparative Study

Enzyme-linked immunosorbent assay (ELISA) in the paracoccidioidomycosis. Comparison with counterimmunoelectrophoresis and erythro-immunoassay

Z P de Camargo et al. Mycopathologia. .

Abstract

An enzyme-linked immunosorbent assay (ELISA) for detection and quantification of antibodies anti-Paracoccidioides brasiliensis is described. Polystyrene plates have been used as solid phase to absorb P. brasiliensis metabolic yeast phase antigen. Twenty sera of proven paracoccidioidomycosis, 11 of histoplasmosis due Histoplasma capsulatum, 20 of aspergillosis and 20 human normal sera were tested. Ninety-five percent of the paracoccidioidomycosis sera had O.D. superior to 0.150 (from 0.163 to 2.650) at 1/400 serum dilution. ELISA assay was compared with counterimmunoelectrophoresis and erythro-immunoassay tests; a correlation was observed only with erythro-immunoassay. ELISA test should give new perspectives for the serodiagnosis of paracoccidioidomycosis.

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References

    1. Rev Inst Med Trop Sao Paulo. 1971 Sep-Oct;13(5):320-7 - PubMed
    1. Mycopathologia. 1980 Mar 17;70(2):89-93 - PubMed
    1. Sabouraudia. 1984;22(1):73-7 - PubMed
    1. Sabouraudia. 1976 Nov;14(3):275-80 - PubMed
    1. Clin Allergy. 1979 Jul;9(4):359-71 - PubMed

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