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. 1984;14(4):303-11.
doi: 10.1080/10826068408070637.

A rapid, high-yield method of producing mono-[125I]A14 iodoinsulin

M N Lioubin, M D Meier, B H Ginsberg

A rapid, high-yield method of producing mono-[125I]A14 iodoinsulin

M N Lioubin et al. Prep Biochem. 1984.

Abstract

We have developed a rapid method for producing homogeneous mono-[125I]A14 iodoinsulin with high specific activity and yield. After iodination by the lactoperoxidase method, the labeled peptides were applied to a C18 Porasil pre-column, washed with aqueous buffer to eliminate the free [125I]-iodide and placed "in-line" with a C-18 HPLC column; mono-[125I]A14 iodoinsulin was then eluted isocratically with 29% acetonitrile in 16 minutes. The labeled hormone was extremely stable, and proved suitable for various biological studies.

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