A novel procedure for labeling immunoglobulins by conjugation to oligosaccharide moieties

Abstract

A novel method is described for the biotinylation of immunoglobulins. The procedure relies on the generation of reactive aldehydes on the carbohydrate moieties of the immunoglobulin by oxidation with sodium periodate and subsequent reaction with biotin hydrazide. The method is simple and specific and results in stable conjugates retaining full immunologic activity. It has been applied successfully to a number of mouse monoclonal antibodies of both IgG and IgM classes, and to human IgM preparations. The procedure may also be applied to conjugation of immunoglobulins with fluorescent dyes.