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. 1978 Apr 15;34(4):427-8.
doi: 10.1007/BF01935903.

Absence of low molecular weight DNA polymerase activity from the nuclei of Amoeba discoides

G F Abbott, S E Hawkins

Absence of low molecular weight DNA polymerase activity from the nuclei of Amoeba discoides

G F Abbott et al. Experientia. .

Abstract

Amoeba discoides nuclear protein partially purified by passage through Sephadex G-200 showed 3 high-mol.-wt. DNA polymerase activities which eluted in and just following the void volume. No low-mol.-wt (45,000 daltons) DNA polymerase beta activity was detected. Nuclear protein layered on 5--20% sucrose gradients also showed an absence of low-mol.-wt DNA polymerase beta. The void volume enzyme showed deoxyribonuclease activity, but no low-mol.-wt nuclease activity was detected.

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