Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Jan;39(1):483-6.
doi: 10.1128/iai.39.1.483-486.1983.

Elaboration of Bacillus anthracis antigens in a new, defined culture medium

J D Ristroph, B E Ivins

Elaboration of Bacillus anthracis antigens in a new, defined culture medium

J D Ristroph et al. Infect Immun. 1983 Jan.

Abstract

Improved culture conditions and a new, completely synthetic medium (R medium) were developed to facilitate the production of Bacillus anthracis holotoxin antigens. Levels of these antigens up to fivefold greater than the highest previously reported values were recovered with the described system. Cultures of Sterne, V770-NP1-R, and Vollum 1B strains of B. anthracis were monitored for growth, pH change, glucose utilization, supernatant protein concentration, lethal toxin activity, and protease activity.

PubMed Disclaimer

References

    1. J Immunol. 1966 Apr;96(4):727-33 - PubMed
    1. J Bacteriol. 1963 May;85:1032-8 - PubMed
    1. Fed Proc. 1967 Sep;26(5):1527-31 - PubMed
    1. Fed Proc. 1967 Sep;26(5):1534-8 - PubMed
    1. J Infect Dis. 1968 Feb;118(1):85-96 - PubMed

MeSH terms

LinkOut - more resources