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. 1983 Feb;17(2):323-6.
doi: 10.1128/jcm.17.2.323-326.1983.

Affinity purification of bovine antibodies to Brucella abortus Lipopolysaccharide

J M Stiller, K H Nielsen

Affinity purification of bovine antibodies to Brucella abortus Lipopolysaccharide

J M Stiller et al. J Clin Microbiol. 1983 Feb.

Abstract

Alkali-treated lipopolysaccharide from Brucella abortus S1119.3 coupled to agarose beads by cyanogen bromide activation resulted in an immunoadsorbent with which a large amount of B. abortus-specific antibodies could be purified. The method described gave alkali-treated lipopolysaccharide binding efficiencies of up to 98%. There was little loss of alkali-treated lipopolysaccharide from the column after several pH shifts, allowing the immunoadsorbent to be regenerated and used repeatedly.

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