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. 1983 Mar;129(2):430-3.
doi: 10.1016/0003-2697(83)90573-0.

A rapid one-step purification of NADPH-cytochrome c (P-450) reductase from rat liver microsomes

E A Shephard, S F Pike, B R Rabin, I R Phillips

A rapid one-step purification of NADPH-cytochrome c (P-450) reductase from rat liver microsomes

E A Shephard et al. Anal Biochem. 1983 Mar.

Abstract

NADPH-cytochrome c (P-450) reductase from liver microsomes of phenobarbital-treated rats has been purified in a single step by affinity chromatography on agarose-hexane-adenosine 2',5'-diphosphate. As determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, enzyme assay, and radioimmunoassay the protein obtained by this single step procedure is as pure as that isolated by multicolumn procedures.

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