Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Apr 1;30(1):71-9.
doi: 10.1016/0049-3848(83)90398-5.

Human and rabbit platelets form platelet-activating factor in response to calcium ionophore

I Alam, J B Smith, M J Silver

Human and rabbit platelets form platelet-activating factor in response to calcium ionophore

I Alam et al. Thromb Res. .

Abstract

Homogenous preparations of human and rabbit platelets synthesized platelet-activating factor (PAF) when stimulated by calcium ionophore A23187. PAF was isolated by high performance liquid chromatography (HPLC) on a Lichrosorb Si60 column. PAF was well separated from other phospholipids especially sphingomyelin and lysolecithin. The retention times of PAF derived from human or rabbit platelets were identical to the commercially available semisynthetic PAF and radiolabeled PAF. The amount of PAF was determined by the extent to which it induced the aggregation of washed rabbit platelets.

PubMed Disclaimer

LinkOut - more resources