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. 1983 Apr 15;212(1):211-8.
doi: 10.1042/bj2120211.

Sugar transport in rat liver lysosomes. Direct demonstration by using labelled sugars

G A Maguire, K Docherty, C N Hales

Sugar transport in rat liver lysosomes. Direct demonstration by using labelled sugars

G A Maguire et al. Biochem J. .

Abstract

Purified rat liver lysosomes ('tritosomes') were prepared from rats injected with Triton WR-1339. 2. The water space of tritosomes, measured by using [3H]water and [14C]sucrose, was 2.15 +/- 0.72 microliter/mg of protein (mean +/- S.E.M., n = 12). 3. Tritosomes, when compared with a crude preparation of normal lysosomes by an indirect method of study, showed sugar specificity but decreased stereospecificity of sugar uptake. 4. At 125 mM the relative rates of net uptake of D-[14C]ribose, D-[14C]- or D-[3H]glucose and 2-deoxy-D-[3H]glucose were the same as that inferred from the indirect study. 5. The entry of D-[3H]glucose into tritosomes showed concentration-dependence suggestive of saturation, with a Km of 48 +/- 18 mM (4). 6. D- and L-glucose, D-ribose, 2-deoxy-D-glucose and D-mannose competed with D-[14C]glucose or D-[14C]ribose for uptake. 7. Cytochalasin B inhibited D-[3H]glucose uptake. 8. Uptake of 1 mM-L-[14C]glucose was slower than for 1 mM-D-[14C]glucose. 9. It is concluded that a facilitated-diffusion transport system is present in purified rat liver lysosomes.

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References

    1. J Cell Biol. 1968 May;37(2):482-513 - PubMed
    1. Biochim Biophys Acta. 1971 Apr 13;233(2):334-47 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1430-4 - PubMed
    1. Biochim Biophys Acta. 1972 Jul 13;276(1):192-205 - PubMed
    1. Methods Enzymol. 1974;31:323-9 - PubMed

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